名称 | HC11 (小鼠乳腺上皮细胞) (STR鉴定正确) |
别称 | HC-11; HC11 Mammary Epithelium |
种属 | 小鼠 |
年龄(性别) | 雌性;1岁 |
组织来源 | 乳腺 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
背景描述 | This clone was isolated by transfection from the parental cell line COMMA-1D. It was selected for its prolactin induction of beta-casein protein. |
生物安全等级 | 1 |
生长培养基 | RPMI-1640+10% FBS+1%P/S |
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2~3次/周 |
倍增时间 | ~50-80 hours (DSMZ) |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5%温度:37℃ |
基因表达情况 | Undifferentiated cells express Lgals1, Ran, Jam-A and Bmpr1a Differentiated cells express Id1, Nfkbiz, Trib 1, Rps21, ler3 |
保藏机构 | ATCC; CRL-3062 DSMZ; ACC-780 |