名称 | HL-1 (小鼠心肌细胞) (种属鉴定正确) |
别称 | HL1 |
种属 | 小鼠 |
年龄(性别) | 雌 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
背景描述 | HL-1 Cardiac Muscle Cell Line is an immortalized mouse cardiomyocyte cell line able to continuously divide and spontaneously contract while maintaining a differentiated cardiac phenotype. HL-1 can be serially passaged without losing its differentiated cardiac myocyte phenotype, including morphological, biochemical, and electrophysiological properties. HL-1 is used in a variety of model systems to address questions of cardiac biology at the cellular & molecular levels. |
生物安全等级 | 1 |
生长培养基 | MEM+10% FBS+1% P/S |
推荐传代比例 | 1:4-1:6 |
推荐换液频率 | 2~3次/周 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5%温度:37℃ |