名称 | LS513 (人结直肠癌细胞) (STR鉴定正确) |
别称 | LS-513; LS 513 |
种属 | 人 |
年龄(性别) | 男;63岁 |
组织来源 | 盲肠 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
背景描述 | LS513 is a colorectal carcinoma cell line isolated in 1985 from a primary tumor biopsy from a 63 year old Caucasian male patient diagnosed with a Dukes' C mucin secreting cecal tumor located at the Bauhin valve. |
生物安全等级 | 1 |
生长培养基 | RPMI-1640+10% FBS+1%P/S |
推荐传代比例 | 1:3-1:5 |
推荐换液频率 | 2~3次/周 |
倍增时间 | 20 hours (PubMed=25984343) |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5%温度:37℃ |
致瘤性 | Yes, forms tumors in nude mice |
抗原表达情况 | carcinoembryonic antigen (CEA); ICAM-1; HLA class I positive |
基因表达情况 | transforming growth factor beta 1 |
保藏机构 | ATCC; CRL-2134 |