名称 | KYSE-150 (人食管鳞癌细胞) (STR鉴定正确) |
别称 | KYSE 150; KYSE150; Kyse150; KY150 |
种属 | 人 |
年龄(性别) | 女;49岁 |
组织来源 | 食管 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
背景描述 | Established from the poorly differentiated esophageal squamous cell carcinoma resected from upper (cervical) esophagus of a 49-year-old Japanese woman after receiving radiotherapy (tumor was invading contiguous structures); described as carrying amplified oncogenes, c-erb-B (8x) and cyclin D1 (4x) and producing tumors in nude mice. |
生物安全等级 | 1 |
生长培养基 | RPMI-1640+Ham's F-12+10% FBS+1%P/S |
推荐传代比例 | 1:4-1:8 |
推荐换液频率 | 2~3次/周 |
倍增时间 | 13.7 hours (PubMed=1728357); 23 hours (PubMed=25984343); <25 hours, less when serum amount is increased (DSMZ) |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5%温度:37℃ |
致瘤性 | Yes, in nude mice |
保藏机构 | DSMZ; ACC-375 |