名称 | SW948 (人结肠腺癌细胞) (STR鉴定正确) |
别称 | SW-948; SW 948 |
种属 | 人 |
年龄(性别) | 女;81岁 |
组织来源 | 结肠 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
背景描述 | Established from the colon tumor of a 81-year-old Caucasian woman with colon adenocarcinoma (Duke C) |
生物安全等级 | 1 |
生长培养基 | Leibovitz's L-15+10% FBS+1%P/S |
推荐传代比例 | 1:2-1:5 |
推荐换液频率 | 2~3次/周 |
倍增时间 | 120 hours, at 8th passage (PubMed=1000501); 2-4 days (DSMZ) |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
培养条件 | 气相:空气,100%温度:37℃ |
致瘤性 | Yes, in nude mice Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
基因表达情况 | carcinoembryonic antigen (CEA) 7 ng/106 cells/10 days; colon specific antigen (CSAp) 750 units in 0.5 mL cell sonicate; keratin |
保藏机构 | ATCC; CCL-237 DSMZ; ACC-326 ECACC; 91030714 |