名称 | MIA PaCa-2 (人胰腺癌细胞) (STR鉴定正确) |
别称 | MIA-PaCa-2; MIA-PACA-2; MIA-Pa-Ca-2; MIA Paca2; MIA PaCa2; MiaPaCa-2; MIAPACA-2; MiaPaca.2; MiaPaCa2; Miapaca2; MIAPaCa2; MIAPACA2; Mia PACA 2; MIAPaCa-2; PaCa2 |
种属 | 人 |
年龄(性别) | 男;65岁 |
生长特性 | 贴壁/悬浮细胞 |
细胞形态 | 上皮样贴壁细胞,伴有圆形漂浮细胞 |
背景描述 | The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male. |
生物安全等级 | 1 |
生长培养基 | DMEM+10%FBS+2.5%HS+1%P/S |
推荐传代比例 | 1:3-1:8 |
推荐换液频率 | 2~3次/周 |
倍增时间 | 26 hours (PubMed=25984343); 25.7 +- 4.3 hours (PubMed=27067801); 40 hours, 18 hours, in serum-free medium (PubMed=23386380); ~40 hours (ATCC); ~30-40 hours (DSMZ) |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5%温度:37℃ |
基因表达情况 | human colony stimulating factor, subclass I (CSF-I); plasminogen activator |
保藏机构 | ATCC; CRL-1420 DSMZ; ACC-733 ECACC; 85062806 JCRB; JCRB0070 |