名称 | Daoy (人髓母细胞瘤细胞) (STR鉴定正确) |
别称 | DAOY; D324 Med; D-324 Med; D324 MED; D-324MED; D324 |
种属 | 人 |
年龄(性别) | 男;4岁 |
组织来源 | 结缔组织增生性小脑髓母细胞 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
背景描述 | The Daoy cell line was established in 1985 by P. F Jacobsen of the Royal Perth Hospital in Western Australia. The line was derived from biopsy material taken from a tumor in the posterior fossa of a 4 year old boy. |
生物安全等级 | 1 |
生长培养基 | MEM+10% FBS+1%P/S |
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2~3次/周 |
倍增时间 | 33.6 hours, at 48th passage (PubMed=2993532); 34 hours (ATCC) |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5%温度:37℃ |
致瘤性 | Yes, in nude mice (The cells form serially transplantable intercranial and subcutaneous tumors.) |
保藏机构 | ATCC; HTB-186 BCRJ; 0325 |