名称 | MM.1S (人IgA- 骨髓瘤细胞) |
别称 | MM.1S; MM1-S; MM-1S; MM1S |
种属 | 人 |
年龄(性别) | 女;45岁 |
组织来源 | 外周血 |
生长特性 | 悬浮细胞 |
细胞形态 | 淋巴细胞样 |
背景描述 | The parent cell line, MM.1, was established from peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy. The closely related cell line, MM.1R was also isolated from MM.1, but is resistant to dexamethasone. |
生物安全等级 | 1 |
生长培养基 | RPMI-1640+10% FBS+1%P/S |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2~3次/周 |
倍增时间 | 80 hours (PubMed=25984343) |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5%温度:37℃ |
受体表达情况 | glucocorticoid receptor, expressed |
抗原表达情况 | CD25+, CD38+, CD52+, CD59+ |
基因表达情况 | lambda-light chain immunoglobulin |
保藏机构 | ATCC; CRL-2974 |