1600.00
SNU-387 (人肝癌细胞) (STR鉴定正确)
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名称SNU-387 (人肝癌细胞) (STR鉴定正确)
别称SNU387; NCI-SNU-387
种属
年龄(性别)女;41岁
组织来源肝癌
生长特性贴壁细胞
细胞形态上皮细胞样
背景描述SNU-387 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus a combination of doxorubicin and mitomycin-C. Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat inactivated fetal bovine serum.
生物安全等级2
生长培养基RPMI-1640+10% FBS+1% P/S
推荐传代比例1:3-1:6
推荐换液频率2~3次/周
倍增时间61 hours (PubMed=7543080)
冻存条件冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮
培养条件气相:空气,95%;CO2,5%温度:37℃
保藏机构ATCC; CRL-2237


货号
sc-01612
规格
1×10⁶cells/T25培养瓶
价格
1600.00
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