名称 | KYSE-410 (人食管鳞癌细胞) (STR鉴定正确) |
别称 | KYSE 410; KYSE410; KYSE0410 |
种属 | 人 |
年龄(性别) | 女;51岁 |
组织来源 | 食管鳞癌 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
背景描述 | Established from the poorly differentiated invasive esophageal squamous cell carcinoma resected from the cervical esophagus of a 51-year-old Japanese man prior to treatment (tumor invasion into the adventitia was obvious); described in the literature to overexpress hst-1 (= heparin-binding growth factor) and cyclin D1 |
生物安全等级 | 1 |
生长培养基 | RPMI-1640+10% FBS+1% P/S |
推荐传代比例 | 1:4-1:6 |
推荐换液频率 | 2~3次/周 |
倍增时间 | ~32-45 hours |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5%温度:37℃ |
保藏机构 | DSMZ; ACC-381 ECACC; 94072023 JCRB; JCRB1419 |