名称 | SNB-19 (人胶质瘤细胞) (U-251 MG污染细胞系,暂不供应) |
别称 | SNB.19; SNB19 |
种属 | 人 |
年龄(性别) | 男;75岁 |
组织来源 | 星形胶质瘤(U-251MG) |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
背景描述 | Established from the surgical resection of a left parieto-occipital glioblastoma from a 47-year-old man in 1980; cells were described in the literature to secrete plasminogen activator, to be clonogenic in soft agar and to be tumorigenic in nude mice; DNA fingerprinting showed unequivocally that SNB-19 is a subclone of the astrocytoma cell line U-251 MG)。(STR检测位点同U-251MG) |
生物安全等级 | 1 |
生长培养基 | DMEM+10% FBS+1% P/S |
推荐传代比例 | 1:4-1:6 |
推荐换液频率 | 2~3次/周 |
倍增时间 | ~24-36 hours |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5%温度:37℃ |
保藏机构 | DSMZ; ACC-325 |