名称 | BT-B (人膀胱癌细胞) (Hela污染细胞系,暂不供应) |
种属 | 人 |
年龄(性别) | 女;30岁6个月 |
组织来源 | 膀胱癌组织 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
背景描述 | These cells were thought to be "stablished from the malignant urinary bladder carcinoma of a 66-year-old Caucasian man (stage pTa G3) in 1989"; same donor as for cell line BT-A; however, DNA fingerprinting and cytogenetic analysis at the DSMZ Established unequivocally that this cell line must be considered de facto a subclone of HELA。(STR检测位点同HELA) |
生物安全等级 | 1 |
生长培养基 | RPMI-1640+10% FBS+1% P/S |
推荐传代比例 | 1:4-1:6 |
推荐换液频率 | 2~3次/周 |
倍增时间 | ~48 hours |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5%温度:37℃ |
保藏机构 | DSMZ; ACC-227 |