名称 | KYSE-30 (人食管鳞癌细胞) (KYSE-30 (人食管鳞癌细胞) (STR鉴定正确) |
别称 | KYSE 30; KYSE30; KYSE0030 |
种属 | 人 |
年龄(性别) | 女;64岁 |
组织来源 | 食管鳞状上皮 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样,带有长的伪足 |
背景描述 | Derived from well differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 64-year-old Japanese man prior to treatment; cell line Established from tumor cells heterotransplanted into athymic mice; described in the literature to be heterotransplantable in athymic mice and to carry p53 mutation and amplification of cERB B, MYC and CYCLIN D1 |
生物安全等级 | 1 |
生长培养基 | RPMI-1640+Ham's F-12+2mM L-Glutamine+10% FBS+1% P/S |
推荐传代比例 | 1:3-1:5 |
推荐换液频率 | 2~3次/周 |
倍增时间 | ~20-30 hours |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5%温度:37℃ |
保藏机构 | DSMZ; ACC-351 ECACC; 94072011 JCRB; JCRB0188 |