名称 | HPAC (人胰腺腺泡上皮癌) |
别称 | Hpac |
种属 | 人 |
年龄(性别) | 64岁 |
组织来源 | 胰腺 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
生物安全等级 | 1 |
生长培养基 | DMEM/F12+2μg/ml Insulin+5μg/ml Transferrin+40ng/ml Hydrocortisone+10ng/ml EGF+5% FBS+1% P/S |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2~3次/周 |
倍增时间 | ~23-44 hours |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5%温度:37℃ |
致瘤性 | Yes, the cells form tumors in athymic nude mice at the site of inoculation which are histologically similar to the tumor of origin. Yes, the cells have a colony forming efficiency of 64% on plastic and 3.2% in agarose. |
受体表达情况 | epidermal growth factor (EGF), expressed; glucocorticoid, expressed; epidermal growth factor (EGF); glucocorticoid |
基因表达情况 | HPAC cells are positive for keratin and negative for vimentin and chromogranin A. |
保藏机构 | ATCC; CRL-2119 |