名称 | D283 Med (人脑髓母细胞瘤细胞) |
别称 | D283 MED; D283-MED; D-283 Med; D-283MED; D283MED; D283Med; D283-Med; D283; Med 283; H283 |
种属 | 人 |
年龄(性别) | 男;6岁 |
组织来源 | 脑组织 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
背景描述 | D283 Med细胞是由Friedman等人于1985年建系,源自一名6岁患有神经管细胞瘤有腹腔转移的白人男性小孩的腹水细胞。 |
生物安全等级 | 1 |
生长培养基 | MEM+10% FBS+1% P/S |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2~3次/周 |
倍增时间 | ~52 hours |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5%温度:37℃ |
致瘤性 | Yes, in nude mice (The cells form serially transplantable intercranial and subcutaneous tumors). Yes, in soft agar. |
基因表达情况 | The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells express elevated levels of four biochemical markers of SCLC (neuron specific enolase, the brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity. |
保藏机构 | ATCC; HTB-185 |