名称 | RG2 [D74](大鼠胶质瘤细胞) |
别称 | RG-2; Rat Glioma-2; D74; D74-RG2 |
种属 | 褐家鼠 |
年龄(性别) | 孕20天 |
组织来源 | 脑部 |
生长特性 | 贴壁 |
细胞形态 | 成纤维细胞样 |
背景描述 | This cell line may be used for both in vitro and in vivo studies of a rat brain tumor. It grows well in cell culture and provides a simple, reproducible glioma model when inoculated into the brains of syngeneic rats.The RG2 and F98 (ATCC CRL-2397) gliomas can be used as rat brain tumor models in experimental neuro-oncology. |
生物安全等级 | 1 |
生长培养基 | DMEM+10%FBS+1%P/S |
推荐传代比例 | 1:4 - 1:8 |
推荐换液频率 | 2~3次/周 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5%温度:37℃ |
保藏机构 | ATCC; CRL-2433 |